Turbo FISH: A Method for Rapid Single Molecule RNA FISH

Por um escritor misterioso
Last updated 22 dezembro 2024
Turbo FISH: A Method for Rapid Single Molecule RNA FISH
Advances in RNA fluorescence in situ hybridization (RNA FISH) have allowed practitioners to detect individual RNA molecules in single cells via fluorescence microscopy, enabling highly accurate and sensitive quantification of gene expression. However, current methods typically employ hybridization times on the order of 2–16 hours, limiting its potential in applications like rapid diagnostics. We present here a set of conditions for RNA FISH (dubbed Turbo RNA FISH) that allow us to make accurate measurements with no more than 5 minutes of hybridization time and 3 minutes of washing, and show that hybridization times can go as low as 30 seconds while still producing quantifiable images. We further show that rapid hybridization is compatible with our recently developed iceFISH and SNP FISH variants of RNA FISH that enable chromosome and single base discrimination, respectively. Our method is simple and cost effective, and has the potential to dramatically increase the throughput and realm of applicability of RNA FISH.
Turbo FISH: A Method for Rapid Single Molecule RNA FISH
Single-Molecule RNA FISH
Turbo FISH: A Method for Rapid Single Molecule RNA FISH
Paired Capture and FISH Detection of Individual Virions Enable Cell-Free Determination of Infectious Titers
Turbo FISH: A Method for Rapid Single Molecule RNA FISH
FISHing for Accurate Transcript Counts with Single-Molecule FISH
Turbo FISH: A Method for Rapid Single Molecule RNA FISH
ClampFISH 2.0 enables rapid, scalable amplified RNA detection in situ
Turbo FISH: A Method for Rapid Single Molecule RNA FISH
Detection of mRNA Transfer Between Mammalian Cells in Coculture by Single- Molecule Fluorescent In Situ Hybridization (smFISH)
Turbo FISH: A Method for Rapid Single Molecule RNA FISH
Viral detection and identification in 20 min by rapid single-particle fluorescence in-situ hybridization of viral RNA
Turbo FISH: A Method for Rapid Single Molecule RNA FISH
Subcellular Detection of SARS-CoV-2 RNA in Human Tissue Reveals Distinct Localization in Alveolar Type 2 Pneumocytes and Alveolar Macrophages
Turbo FISH: A Method for Rapid Single Molecule RNA FISH
Publications — Raj Lab
Turbo FISH: A Method for Rapid Single Molecule RNA FISH
PDF] Real-Time Monitoring of Fluorescence in Situ Hybridization Kinetics.
Turbo FISH: A Method for Rapid Single Molecule RNA FISH
RNA fluorescence in situ hybridization for high-content screening - ScienceDirect

© 2014-2024 fluidbit.co.ke. All rights reserved.